Liquid Chromatography
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This chapter provides an overview of the most common equipment used for performing high-pressure liquid chromatography (HPLC), under both conventional (up to 6000 psi; 400 bar) and very high pressure conditions (6,000 –20,000 psi; 400-1400 bar), including: pumps, sample injectors, column compartments, and connections. The operation and figures of merit of the most frequently encountered detectors (absorbance, refractive index, nanoparticle based, conductivity, fluorescence, and electrochemical) are also described. Practical tips and considerations for achieving optimal chromatography are provided throughout..